Development of a near-infrared fluorescence ELISA method using tyramide signal amplification |
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Authors: | Gong Haibiao Cradduck Mark Cheung Lael Olive D Michael |
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Affiliation: | LI-COR Biosciences, Lincoln, NE 68504, USA. herbert.gong@licor.com |
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Abstract: | In this study, we applied tyramide signal amplification (TSA) to fluorescence enzyme-linked immunosorbent assay (ELISA) employing horseradish peroxidase (HRP) as the detection enzyme. When used with a human epidermal growth factor ELISA kit, the TSA method led to a >100-fold increase in fluorescence signal intensity in comparison to an unamplified method. It also showed wider dynamic range and better sensitivity compared to a conventional method using tetramethylbenzidine as the HRP substrate. |
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