A trifunctional enzyme with glutathione S-transferase,glutathione peroxidase and superoxide dismutase activity |
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| Authors: | Fei Yan Wen-kui Yang Xin-yang Li Ting-ting Lin Yan-ni Lun Feng Lin Shao-wu Lv Gang-lin Yan Jun-qiu Liu Jia-cong Shen Ying Mu Gui-min Luo |
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| Institution: | 1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, PR China;2. Key Laboratory for Supramolecular Structure and Materials of the Ministry of Education, Jilin University, Changchun 130012, PR China;3. Research Center for Analytical Instrumentation, Zhejiang University, Hangzhou 310058, PR China |
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| Abstract: | Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a Schistosoma japonicum GST (SjGST) and a bifunctional 35-mer peptide with SOD and GPX activities. The engineered protein demonstrated SOD, GPX and GST activities simultaneously. This trifunctional enzyme with SOD, GPX and GST activities is expected to be the best ROS scavenger. |
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| Keywords: | Reactive oxygen species Trifunctional enzyme Glutathione S-transferase Superoxide dismutase Glutathione peroxidase Enzyme mimic Fusion protein Metal incorporation |
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