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2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor |
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| Authors: | Rodrigo Pontiggia Osvaldo Pontiggia Marina Simian Javier M. Montserrat Joachim W. Engels Adolfo M. Iribarren |
| Affiliation: | 1. INGEBI (CONICET),Vuelta de Obligado 2490-(1428), Buenos Aires, Argentina;2. Instituto de Oncología A. H. Roffo, UBA, Av. San Martín 5481, CABA, Argentina;3. Instituto de Ciencias, Universidad Nacional de Gral. Sarmiento, J. M. Gutierrez 1150, Los Polvorines (B1613GSX), Prov. de Bs. As., Argentina;4. Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main, Germany;5. Laboratorio de Biotransformaciones, Universidad Nacional de Quilmes, Roque Saenz Peña 352 (1876) Bernal, Prov. de Bs. As., Argentina |
| Abstract: | A new synthesis of 2′-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5′-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor α protein (ER-α), and transfection experiments in MCF-7 cell line are also presented. |
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