Identification of small molecule inhibitors of telomerase activity through transcriptional regulation of hTERT and calcium induction pathway in human lung adenocarcinoma A549 cells |
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| Authors: | Yerra Koteswara Rao Te-Yu Kao Ming-Fang Wu Jiunn-Liang Ko Yew-Min Tzeng |
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| Affiliation: | 1. Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Wufeng, Taiwan, ROC;2. Institute of Medical and Molecular Toxicology, Chung Shan Medical University, Taichung, Taiwan, ROC;3. Department of Medical Oncology and Chest Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan, ROC;4. School of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC;5. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC |
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| Abstract: | High telomerase activity (TA) is detected in most cancer cells; and thus, TA inhibition by drug or dietary food components is a new strategy for cancer prevention. In this report, we examined the effects of fourteen natural or synthetic compounds on TA in human lung adenocarcinoma A549 cells. The results demonstrated that some of the tested compounds inhibited TA, being 2′-hydroxy-2,3,4′,6′-tetramethoxychalcone (HTMC) was the most potent among tested. In A549 cells, HTMC also inhibited the cell proliferation, decreased the expression of human telomerase reverse transcriptase (hTERT) and sequentially reduced the hTERT promoter. In soft agar assay HTMC treatment reduced the colony formation of A549 cells. Cellular fractionation and immunofluorescence assay demonstrated that there was no translocation of hTERT from nuclei to cytoplasm. Further studies revealed that the release of Ca2+ was the underlying mechanism of suppressed TA and hTERT transcription in A549 cells exposed to HTMC. These in vitro data support the development of HTMC as a therapeutic agent for cancer complications. |
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