Isothermal sensitive detection of microRNA using an autonomous DNA machine recycling output as input |
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Authors: | Atsushi Ogawa |
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Institution: | Senior Research Fellow Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan |
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Abstract: | An autonomous DNA machine recycling the output as the input for isothermal, sensitive, and specific detection of miRNAs has been developed. This machine shows considerably high signal amplification efficiency (~1000-fold) and thus a low detection limit (~20 amol). The machine also shows high specificity, discriminating 50 amol of synthetic miRNA from 100-fold larger amounts of its family member and from 100 ng of unrelated total RNAs. Moreover, it is available for practically detecting natural miRNAs in total RNAs. |
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