| 青霉素酰化酶固定化前后动力学行为的比较 |
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| 引用本文： | 杨海波,苏志国.青霉素酰化酶固定化前后动力学行为的比较[J].生物学杂志,1999,16(2):23-24. |
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| 作者姓名： | 杨海波 苏志国 |
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| 作者单位： | 中科院大连化学物理所，中科院化学冶金所 |
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| 摘 要： | 在优化的固定化条件下，通过戊二醛交联直接将青霉素酰化酶固定化。在优化的环境条件下测定游离酶和两种固定化酶的动力学常数。结果表明，尽管固定化酶的米氏常数增大，但产物抑制作用减弱，裂解青霉素的实验结果表明，固定化酶更适合在工业上应用。
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| 关 键 词： | 青霉素酰化酶 动力学 |
Studies on the Dynamics of Penicillin Acylase in Free and Immobilized State |
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| YANG Haibo YU Xingju QU Tianming.Studies on the Dynamics of Penicillin Acylase in Free and Immobilized State[J].Journal of Biology,1999,16(2):23-24. |
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| Authors: | YANG Haibo YU Xingju QU Tianming |
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| Abstract: | Penicillin acylase was directly immobilized with glutaraldehyde as the crosslinked agent under the optimal immobilized conditions.The dynamic constans of free enzyme and two kinds of immobilized enzymes were determined under the optimal environmental factors.The results showed that Km of immobilized enzyme was obviouslt increased,but the inhibitions of products were decreased.The experiments of hydrolized penicillin-G showed that immobilized enzyme was suitable for utilization on industrial scale.. |
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| Keywords: | penicillin acylase dynamics |
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