| PCR定点突变血红蛋白基因的克隆和筛选 |
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| 引用本文： | 张浩,王全立.PCR定点突变血红蛋白基因的克隆和筛选[J].生物学杂志,1999,16(6):25-27. |
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| 作者姓名： | 张浩 王全立 |
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| 作者单位： | 军事医学科学院放射医学研究所!北京100850(张浩，毛秉智)，军事医学科学院野战输血研究所!北京100850(王全立，冯起，杜芝燕，刘晓达) |
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| 摘 要： | 为开展以基因重组血红蛋白为基础的血液代用品研究,我们首先用PCR 突变法扩增出含血红蛋白α、β珠蛋白的串联基因片段,经测序表明,所克隆的α、β基因符合预期设计结果,未发生意外突变。
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| 关 键 词： | 多聚酶链式反应 血红蛋白 定点突变 |
Clone and Selection of Mutant Hemoglobin Gene with PCR Site-Directed Mutagenesis |
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| ZHANG Hao,MAO Bing-zhi,WANG Quan-Li ,FENG Qi ,DU Zhi-Yan ,LIU Xiao-Da Institute of Radiation Medicine,Acadamic of Military Medical Sciences Institute of Transfusion Medicine,Acadmic of Military Medical Sciences.Clone and Selection of Mutant Hemoglobin Gene with PCR Site-Directed Mutagenesis[J].Journal of Biology,1999,16(6):25-27. |
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| Authors: | ZHANG Hao MAO Bing-zhi WANG Quan-Li FENG Qi DU Zhi-Yan LIU Xiao-Da Institute of Radiation Medicine Acadamic of Military Medical Sciences Institute of Transfusion Medicine Acadmic of Military Medical Sciences |
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| Institution: | ZHANG Hao,MAO Bing-zhi,WANG Quan-Li 1,FENG Qi 1,DU Zhi-Yan 1,LIU Xiao-Da 1 Institute of Radiation Medicine,Acadamic of Military Medical Sciences Institute of Transfusion Medicine,Acadmic of Military Medical Sciences |
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