Taxon-specific PCR primers to detect two inconspicuous arbuscular mycorrhizal fungi from temperate agricultural grassland |
| |
Authors: | Hannes Gamper Adrian Leuchtmann |
| |
Institution: | 1.Institute of Integrative Biology,ETH Zurich,Zürich,Switzerland;2.Department of Biology,University of York,York,UK |
| |
Abstract: | Taxon-specific polymerase chain reaction (PCR) primers enable detection of arbuscular mycorrhizal fungi (AMF, Glomeromycota)
in plant roots where the fungi lack discriminative morphological and biochemical characters. We designed and validated pairs
of new PCR primers targeted to the flanking regions of the variable domain 1 of the nuclear ribosomal large subunit RNA gene
to specifically detect Acaulospora paulinae and an undescribed member of the Diversisporaceae. These two fungal taxa, sporulating late in soil-trap cultures and showing
small, faintly coloured spores and weakly staining intraradical structures, were frequently found in roots of Trifolium repens from a high-input agricultural grassland. The newly developed PCR primers may thus enable studies on two inconspicuous AMF
taxa that appear to have been overlooked in previous molecular AMF community analyses and for which no specific PCR primers
have been published. |
| |
Keywords: | Acaulospora paulinae Arbuscular mycorrhizal fungi Diversisporaceae Nuclear ribosomal large subunit (LSU 28S) Trifolium repens (white clover) |
本文献已被 PubMed SpringerLink 等数据库收录! |
|