| 苦瓜多糖的提取、分离纯化及组成性质 |
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| 引用本文： | 吴勋贵,王泽根,蔡寅,杨烨,王旻. 苦瓜多糖的提取、分离纯化及组成性质[J]. 生物加工过程, 2011, 9(1): 19-23. DOI: 10.3969/j.issn.1672-3678.2011.01.005 |
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| 作者姓名： | 吴勋贵 王泽根 蔡寅 杨烨 王旻 |
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| 作者单位： | 中国药科大学,生命科学与技术学院,南京,210009 |
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| 基金项目： | 江苏省六大人才高峰资助项目 |
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| 摘 要： | 正交实验确定提取工艺后,用热水提取法得到苦瓜多糖(MCP).对MCP进行DEAE-32离子交换层析分离,得到3个多糖组分MCP1、MCP2和MCP3. 进一步采用Sephacryl S-400凝胶层析进行分离,经凝胶层析和高效液相色谱检测表明,MCP1、MCP2为均一性多糖组分.通过高效液相凝胶色谱法测定了两者的相对分子质量分别为1.16×106和7.45×105.用PMP衍生化法测定其单糖,结果表明: MCP1系由Man、Rham、GlcUA、GalUA、Glu、Gal、Xyl、Ara等单糖组成的杂多糖,摩尔比为1.03:2.93:1.00:14.95:2.16:30.70:2.85:4.50.MCP2系由Rham、GalUA、Gal、Xyl、Ara等单糖组成的杂多糖,对应的摩尔比为1.63:21.88:4.66:1.00:1.29.紫外光谱表明该多糖不含蛋白质和核酸.
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| 关 键 词： | 苦瓜多糖 分离纯化 单糖 衍生化 |
Extraction, isolation, purification and composition of Momordica charantia polysaccharide |
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| WU Xungui,WANG Zegen,CAI Yin,YANG Ye,WANG Min. Extraction, isolation, purification and composition of Momordica charantia polysaccharide[J]. Chinese Journal of Bioprocess Engineering, 2011, 9(1): 19-23. DOI: 10.3969/j.issn.1672-3678.2011.01.005 |
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| Authors: | WU Xungui WANG Zegen CAI Yin YANG Ye WANG Min |
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| Affiliation: | Zegen,CAI Yin,YANG Ye,WANG Min(College of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China) |
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| Abstract: | Crude polysaccharides were extracted from Momordica charantia with hot water.Three main fractions of Momordica charantia polysaccharides（MCP）： MCP1,MCP2 and MCP3 were obtained through purification on DEAE-32 ion exchange column.The fractions were further isolated by Sephacryl S-400 gel-permeation chromatography.The results by Sephacryl S-400 and HPLC showed that MCP1 and MCP2 were both homogeneous.The molecular weight of MCP1 and MCP2 were 1.16×10 6 and 7.45×105,respectively measured by high performance gel permeation chromatography.The components of MCPs were determined by HPLC after PMP-derivatization.The results showed that MCP1 was heteropolysaccharides composed of Man,Rham,GlcUA,GalUA,Glu,Gal,Xyl and Ara,with respective molar ratios of 1.03∶2.93∶1.00∶14.95∶2.16∶30.70∶2.85∶4.50.MCP2 was composed of Rham,GalUA,Gal,Xyl and Ara,with respective molar ratios of 1.63∶21.88∶4.66∶1.00∶1.29.No typical absorption of nucleic acid or protein was detected by UV scanning. |
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| Keywords: | Momordica charantia polysaccharide isolation and purification monosaccharide derivatization |
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