| 澳洲宝石鲈线粒体DNA COI基因的克隆与序列分析 |
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| 引用本文： | 张龙岗,郭金峰,孟庆磊,安丽,董学飒,付佩胜.澳洲宝石鲈线粒体DNA COI基因的克隆与序列分析[J].生物技术通报,2011(4). |
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| 作者姓名： | 张龙岗 郭金峰 孟庆磊 安丽 董学飒 付佩胜 |
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| 作者单位： | 山东省淡水水产研究所山东省淡水水产遗传育种重点实验室,济南,250117 |
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| 基金项目： | 山东省农业良种工程项目 |
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| 摘 要： | 对澳洲宝石鲈线粒体DNA细胞色素氧化酶Ⅰ亚基(cytochrome oxidase subunit Ⅰ,COI)基因进行了扩增、克隆和测序,并对COI序列进行了分析.结果显示,澳洲宝石鲈COI基因序列长度为631 bp,其中A、T、G和C 4种碱基的含量分别为27.7%,23.6%,29.8%和18.9%.与从GenBank中查到的其他4种蜊科鱼类的同源序列比对,邻接法(neighbor-joining)构建系统进化树.结果显示,5种蜊科鱼类聚在一起,分为两个大的支系,其中花身蜊与条纹蜊首先聚成一支,然后与詹氏弱棘鯻和银锯眶鯻聚成的一支共同组成一个支系;而高体革鯻单独聚为一个支系,所得的聚类结果与传统的分类结果基本一致.
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| 关 键 词： | 宝石鲈 克隆 序列分析 |
Cloning and Sequencing Analysis of COI Gene from Scortum barcoo |
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| Zhang Longgang,Guo Jinfeng,Meng Qinglei,An Li,Dong Xuesa,Fu Peisheng.Cloning and Sequencing Analysis of COI Gene from Scortum barcoo[J].Biotechnology Bulletin,2011(4). |
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| Authors: | Zhang Longgang Guo Jinfeng Meng Qinglei An Li Dong Xuesa Fu Peisheng |
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| Institution: | Zhang Longgang Guo Jinfeng Meng Qinglei An Li Dong Xuesa Fu Peisheng(Fresh Water Fisheries Research Institute of Shandong Province,Shandong Provincial Key Lab for Genetics & Breeding of Freshwater Fisheries,Jinan 250117) |
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| Abstract: | Mitochondrial COI gene fragment of Scortum barcoo was amplified via PCR,then the PCR products were purified,cloned and sequenced.For the COI gene fragment,631 bp nucleotide sequences were obtained,and the A,T,G and C contents were 27.7%,23.6%,29.8%and 18.9%.Found four species of Teraponidae from the GenBank and alignmented the sequences,clustering analysis could build phylogenetic tree with neighbor-joining of the five species.It was revealed that the five species clustered together and divided into two big... |
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| Keywords: | COI |
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