| 葡萄糖3-脱氢酶产生菌的筛选和产酶培养 |
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| 引用本文： | 张建芬,沈明杰,曾为锋,季佳,连振静. 葡萄糖3-脱氢酶产生菌的筛选和产酶培养[J]. 生物加工过程, 2011, 9(1): 41-45. DOI: 10.3969/j.issn.1672-3678.2011.01.009 |
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| 作者姓名： | 张建芬 沈明杰 曾为锋 季佳 连振静 |
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| 作者单位： | 浙江树人大学,生物与环境工程学院,杭州,310015 |
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| 基金项目： | 浙江省大学生科技成果推广项目(2009)，浙江树人大学人才引进科研项目 |
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| 摘 要： | 从土壤中筛选分离得到1株葡萄糖3-脱氢酶产生菌,经生理生化初步鉴定为产酸克雷伯菌(Klebsiella oxyto-ca)。将菌株D8进行产酶优化培养,经过单因素法确定优化培养条件:乳糖为C源,酵母膏为N源,250 mL摇瓶装液量为50 mL,培养基的初始pH为7,33℃培养时G3DH酶比活力最高,达到1.45 U。
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| 关 键 词： | 葡萄糖3-脱氢酶 产酶培养 Klebsiella oxytoca |
Screening of glucoside 3-dehydrogenase producing strains and its fermentable condition |
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| ZHANG Jianfen,SHEN Mingjie,ZENG Weifeng,JI Jia,LIAN Zhenjing. Screening of glucoside 3-dehydrogenase producing strains and its fermentable condition[J]. Chinese Journal of Bioprocess Engineering, 2011, 9(1): 41-45. DOI: 10.3969/j.issn.1672-3678.2011.01.009 |
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| Authors: | ZHANG Jianfen SHEN Mingjie ZENG Weifeng JI Jia LIAN Zhenjing |
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| Affiliation: | (College of Biology and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China) |
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| Abstract: | A glucoside 3-dehydrogenase producing was isolated from soil.The strain was identified as Klebsiella oxytoca.The culture condition was optimized by single factor experimental design.The activity of glucoside 3-dehydrogenase produced by this strain was 1.45 U under liquid fermentation conditions： carbon source,lactose,nitrogen source,yeast extract,50 mL culture medium in 250 mL shaking flask at 33 ℃,pH 7.0. |
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| Keywords: | Klebsiella oxytoca |
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