| 桔梗多糖的提取与纯化 |
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| 引用本文： | 贾林,沃兴德,陆金健,王蜜蜜,朱晓松.桔梗多糖的提取与纯化[J].生物学杂志,2011,28(2):21-24. |
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| 作者姓名： | 贾林 沃兴德 陆金健 王蜜蜜 朱晓松 |
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| 作者单位： | 浙江中医药大学生命科学学院,杭州,310053 |
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| 摘 要： | 中药桔梗饮片经水浸提、乙醇分步沉淀,获得乙醇终浓度60%和80%的两个粗多糖组分,分别命名为PPS60和PPS80。经Molish反应、Fehling试剂反应呈阳性,碘-碘化钾反应呈阴性,说明PPS60和PPS80中均含有多糖和还原糖,但不含有淀粉。运用苯酚-硫酸法测得PPS60和PPS80的总糖含量分别为82.25%和84.17%;3,5-二硝基水杨酸（DNS）法测得还原糖含量分别为6.21%和8.05%。PPS60和PPS80经过Sevage法除蛋白、透析、浓缩和Sephrose 6B凝胶柱层析获得两个单一组分。这些结果为进一步研究桔梗多糖奠定了基础。
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| 关 键 词： | 多糖 分步沉淀 Sephrose 6B 纯化 |
Extraction and purification of polysaccharides from Platycodon grandiflorum A.DC |
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| JIA Lin,WO Xing-de,LU Jin-jian,WANG Mi-mi,ZHU Xiao-song.Extraction and purification of polysaccharides from Platycodon grandiflorum A.DC[J].Journal of Biology,2011,28(2):21-24. |
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| Authors: | JIA Lin WO Xing-de LU Jin-jian WANG Mi-mi ZHU Xiao-song |
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| Institution: | (College of Life Sciences,Zhejiang Chinese Medicine University,Hangzhou 310053,China) |
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| Abstract: | Two kinds of crude polysaccharides named PPS60 and PPS80 were isolated from Platycodon grandiflorum A.DC via water-ethanol fractional precipitation.Both of them contained reducing sugar without starch determined by Fehling reaction and iodine-potassium iodide assay.The total sugar contents of PPS60 and PPS80 were 82.25% and 84.17% respectively,detected by the method of phenol-vitriolic test.While by using 3,5-dinitrosalicylic acid（DNS） colorimetry assay,the reducing sugar contents of PPS60 and PPS80 were 6.21% and 8.05% respectively.PPS60 and PPS80 were then purified through Sevage reagents,dialysis,concentration and Sephrose 6B column chromatography and two single fractions were obtained.These results provide basement for further study of polysaccharides from platycodon grandiflorum A.DC. |
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| Keywords: | Sephrose 6B |
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