| 具有抑菌作用的放线菌GNF1的分离鉴定和生物学活性的研究 |
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| 引用本文： | 王磊,孙晗笑,李秀英,莫雪梅,张光. 具有抑菌作用的放线菌GNF1的分离鉴定和生物学活性的研究[J]. 生物技术通报, 2011, 0(4) |
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| 作者姓名： | 王磊 孙晗笑 李秀英 莫雪梅 张光 |
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| 作者单位： | 暨南大学药学院基因组药物研究所,广州,510632 |
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| 基金项目： | 广州市重大科技计划资助项目 |
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| 摘 要： | 从几种复合微生物有机肥中分离出一系列不同的菌株,与实验室保存的菌株GNW(自生固氮菌)和HP2(解磷菌)混合接种培养,检测是否因基因杂交、突变等原因而产生具有抑菌作用的菌株.结果分离出一株具有抑菌作用的放线菌菌株GNF1,根据其形态学特征、生理生化特征和基于16S rRNA基因序列的系统发育分析结果,鉴定其属于链霉菌属(Streptomyces)的一个菌株,GNF1菌株的代谢产物中存在具有抑菌作用的活性成分,与植物根际促生菌GNW、HP2以及某些原核病原微生物共培养培养时能明显抑制它们的生长.
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| 关 键 词： | 根际促生菌 原核病原菌 共培养 代谢产物 抑菌作用 |
Isolation, Identification and Biological Activity of Actinomycete GNF1 Exhibiting Bacteriostasis Activity |
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| Wang Lei,Sun Hanxiao,Li Xiuying,Mo Xuemei,Zhang Guang. Isolation, Identification and Biological Activity of Actinomycete GNF1 Exhibiting Bacteriostasis Activity[J]. Biotechnology Bulletin, 2011, 0(4) |
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| Authors: | Wang Lei Sun Hanxiao Li Xiuying Mo Xuemei Zhang Guang |
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| Affiliation: | Wang Lei Sun Hanxiao Li Xiuying Mo Xuemei Zhang Guang(Institute of Genomic Medicine,College of Pharmacy,Jinan University,Guangzhou 510632) |
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| Abstract: | A series of different strains from compound organic fertilizers were isolated,then cultivated with the GNW and HP2 which persevered by our lab,to detect acteriostasis.The isolated bacterial strain was identified by morphological,physiological and biochemical characters,respectively,and 16S rRNA of the strain was sequenced.The antibacterial activity mensuration of fermenting liquor was to detect bacteriostasis activity.The results of identification demonstrated that an actinomycete GNF1 exhibiting bacteriost... |
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| Keywords: | Growth-promoting rhizobacteria Prokaryotic pathogenic microorganism Co-cultivate Metabolic products Bacteriostasis |
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