| 小鼠骨髓源树突状细胞体外诱导培养及初步鉴定 |
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| 引用本文： | 刘铮,代继宏,符州,冯琳琳.小鼠骨髓源树突状细胞体外诱导培养及初步鉴定[J].生物学杂志,2011,28(2):25-27,31. |
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| 作者姓名： | 刘铮 代继宏 符州 冯琳琳 |
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| 作者单位： | 1. 儿童发育疾病研究省部共建教育部重点实验室儿科学重庆市重点实验室重庆市(儿童发育重大疾病诊治与预防)国防科技合作基地
2. 重庆医科大学附属儿童医院呼吸中心,重庆,400014 |
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| 基金项目： | 重庆市科委自然科学基金 |
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| 摘 要： | 用重组小鼠粒细胞-巨噬细胞集落刺激因子（rmGM-CSF）和重组小鼠白细胞介素4（rmIL-4）体外诱导小鼠骨髓细胞分化为树突状细胞,进行形态学变化观察,分析细胞表面分子,刺激T细胞增殖,探讨小鼠骨髓源树突状细胞（BMDC）体外诱导培养并进行初步鉴定。体外培养9d后BMDC可达80%以上,光镜下可见典型的树突状细胞形态。清楚表达成熟期主要表面标志物,可显著刺激同种异体混合淋巴细胞增殖。获得了较高纯度的BMDC,避免了使用传统磁珠分离方法所带来的成本高,操作复杂,产出率低的弊端,为研究BMDC功能以及运用开展下游实验提供材料。
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| 关 键 词： | 树突状细胞 体外诱导培养 初步鉴定 |
Culture and identification of mouse bone marrow-derived dendritic cells in vitro |
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| LIU Zheng,DAI Ji-hong,FU Zhou,FENG Lin-lin.Culture and identification of mouse bone marrow-derived dendritic cells in vitro[J].Journal of Biology,2011,28(2):25-27,31. |
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| Authors: | LIU Zheng DAI Ji-hong FU Zhou FENG Lin-lin |
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| Institution: | 1.Ministry of Education key laboratory of child development and disorders,Key laboratory of pediatrics in Chongqing,CSTC2009CA5002,Chongqing international science and technology cooperation center for child development and disorders;2.Center of Respiratory Diseases,Children′s Hospital of Chongqing Medical University,Chongqing 400014,China) |
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| Abstract: | To establish a method of cultivation and purification of dendritic cells（DC） from mouse bone marrow in vitro and observe their morphology,recombinant mouse granulocyte and macrophage colony stimulus factor（GM-CSF） and interleukin-4（IL4） induction were used to induce mouse bone marrow cells to form dendritic cells in vitro.After 9 days,the percentage of the cultured DCs was more than 80% with typical morphology of DCs under light microscope.The mature cells had clear marker on their surface.This meant that these used substances could stimulate allogenic mixed lymphocyte proliferation. |
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| Keywords: | dendritic cells culture in vitro initial appraisal |
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