| 寡肽转运蛋白PepT2原核表达质粒的构建及其表达初探 |
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| 引用本文： | 赵东欣,朱其丛,薛永亮,卢奎. 寡肽转运蛋白PepT2原核表达质粒的构建及其表达初探[J]. 生物技术通报, 2011, 0(4) |
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| 作者姓名： | 赵东欣 朱其丛 薛永亮 卢奎 |
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| 作者单位： | 1. 河南工业大学化学化工学院,郑州,450001
2. 河南工业大学化学化工学院,郑州450001;河南工程学院,郑州451191 |
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| 基金项目： | 国家自然科学基金项目，河南省创新型科技人才队伍建设工程项目，郑州市创新型科技人才队伍建设工程资助项目 |
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| 摘 要： | 寡肤转运蛋白(PepT2,peptide transporter,SLC15A2)是哺乳动物体内能够转运二肤、三肽的蛋白.研究表明,一些类肽的小分子药物也是PepT2的底物,但PepT2的结构与生物学功能尚待研究.建立稳定表达PepT2的表达体系是研究PepT2的重要环节.根据GenBank中人PepT2基因序列,借助Primer5.0设计了1对寡核苷酸引物,经PCR合成长达2 190bp的目的序列,通过重组构建pET30a(+)/PepT2表达质柱,测序分析确认目的基因中的3个碱基发生突变.初步研究了pET30a(+)/PepT2在大肠杆菌BL21(DE3)pLysS中的表达,为PepT2原核表达的进一步科研和实际应用奠定了基础.
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| 关 键 词： | 原核表达 基因 |
Plasmid Constuction and Preliminary Prokaryotic Expression of PepT2 |
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| Zhao Dongxin,Zhu Qicong,Xue Yongliang,Lu Kui. Plasmid Constuction and Preliminary Prokaryotic Expression of PepT2[J]. Biotechnology Bulletin, 2011, 0(4) |
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| Authors: | Zhao Dongxin Zhu Qicong Xue Yongliang Lu Kui |
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| Affiliation: | Zhao Dongxin1 Zhu Qicong1 Xue Yongliang1 Lu Kui1,2(1School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001,2Henan Institute of Engineering,Zhengzhou 451191) |
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| Abstract: | PepT2 can transport dipeptide,tripeptide and some small molecule peptide-like drugs,but its structure and biological function need to be investigated.It is important to constuct a stable prokaryotic expression system of PepT2 in investigation of PepT2.A pair of primers was designed according to the hPepT2 gene with Primer5.0 software.The gene sequence of hPepT2 was synthesied by PCR and cloned into the expression vector pET30a(+).The recombinant plasmid pET30a(+)/PepT2 was identified and analyzed,and three ... |
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| Keywords: | PepT2 |
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