Mitochondria and apicoplast of Plasmodium falciparum: behaviour on subcellular fractionation and the implication |
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Authors: | Kobayashi Tamaki Sato Shigeharu Takamiya Shinzaburo Komaki-Yasuda Kanako Yano Kazuhiko Hirata Ayami Onitsuka Izumi Hata Masayuki Mi-ichi Fumika Tanaka Takeshi Hase Toshiharu Miyajima Atsushi Kawazu Shin-ichiro Watanabe Yoh-ichi Kita Kiyoshi |
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Institution: | Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan. |
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Abstract: | The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques--Percoll density gradient centrifugation and fluorescence-activated organelle sorting--for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium. |
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