Isolation and characterization of a <Emphasis Type="Italic">Bacillus licheniformis</Emphasis> strain capable of degrading zearalenone |
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| Authors: | Ping-Jung Yi Cheng-Kang Pai Je-Ruei Liu |
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| Institution: | (1) Institute of Biotechnology and Department of Animal Science and Technology, National Taiwan University, 4F., No. 81, Chang-Xing Street, Taipei, Taiwan;(2) Department of Life Science, National Taiwan Normal University, Taipei, Taiwan;(3) Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan; |
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| Abstract: | The worldwide contamination of cereals, oilseeds, and other crops by mycotoxin-producing moulds is a significant problem.
Mycotoxins have adverse effects on humans and animals that result in illnesses and economic losses. Reduction or elimination
of mycotoxin contamination in food and feed is an important issue. This study aimed to screen soil bacteria for degradation
of zearalenone (ZEN). A pure culture of strain CK1 isolated from soil samples showed most capable of degradation of ZEN. Using
physiological, biochemical, and 16S rRNA gene sequence analysis methods, CK1 was identified as Bacillus licheniformis. Addition of 2 ppm of ZEN in Luria–Bertani (LB) medium, B. licheniformis CK1 decreased 95.8% of ZEN after 36 h of incubation. In ZEN-contaminated corn meal medium, B. licheniformis CK1 decreased more than 98% of ZEN after 36 h of incubation. In addition, B. licheniformis CK1 was non-hemolytic, non-enterotoxin producing, and displayed high levels of extracellular xylanase, cellulase, and protease
activities. These findings suggest that B. licheniformis CK1 could be used to reduce the concentrations of ZEN and improve the digestibility of nutrients in feedstuffs simultaneously. |
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