Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive |
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Authors: | Dong-Hyun Kim Byung-Chul Oh Won-Chan Choi Jung-Kee Lee Tae-Kwang Oh |
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Institution: | (1) Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, 305-600, Korea |
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Abstract: | Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl2 significantly broadened the active pH range and also increased the pH stability of DS11 phytase. |
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Keywords: | calcium pH stability phosphate inhibition phytase thermal stability |
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