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Immunohistochemical detection of adenohypophyseal cells containing hormones in Rana ridibunda
Authors:Dr F Gracia-Navarro  J Doerr-Schott
Institution:(1) Cátedra de Citología e Histología Animal y Vegetal, Facultad de Ciencias, Universidad de Córdoba, Córdoba, Spain;(2) Laboratoire de Cytologie Animale, Institut de Zoologie et de Biologie Générale, Université Louis Pasteur, Strasbourg, France;(3) Cátedra Citología e Histología, Facultad de Ciencias, Universidad de Córdoba, Avda. Medina Azahara, s/n., Córdoba, Spain
Abstract:Summary The pars distalis of the pituitary of Rana ridibunda captured throughout the spring and summer was examined with immunofluorescence techniques using antisera to mammalian pituitary hormones. On the basis of their immunoreactivity, four different cell types are recognized: 1) cells immunoreactive to anti-bovine LTH, 2) cells immunoreactive to anti-bovine STH, 3) cells immunoreactive to anti-ovine LH, and 4) cells immunoreactive to anti-synthetic ACTHbeta (1–24). Their distribution and morphology as well as their staining characteristics (classical histological techniques) are reported in this study.
Keywords:Pituitary hormones  Rana ridibunda  Immunofluorescence microscopy
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