Transformation and expression of a cloned δ-endotoxin gene in bacillus thuringiensis |
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| Authors: | D Lereclus O Arantès J Chaufaux M-M Lecadet |
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| Institution: | Unitéde Biochimie Microbienne, Insitut Pasteur, Paris Cedex 15, France;Station de Recherches de Lutte Biologique, I.N.R.A., La Minière, Guyancourt, France |
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| Abstract: | Abstract A shuttle vector containing the replication region of a resident plasmid of B. thuringiensis , was used to determine the conditions allowing efficient transformation of B. thuringiensis by electroporation. Using this plasmid a δ-endotoxin gene was cloned and expressed both in Escherichia coli and B. thuringiensis . It was shown that this gene was poorly expressed in the wild type situation whereas after cloning in acrystalliferous strains of B. thuringiensis large amounts of crystal protein were obtained. |
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| Keywords: | Electroporation Genetic expression Insecticidal activity Plasmid Toxin gene |
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