Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization |
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| Authors: | Enric Llobet-Brossa Ramon Rossell-Mora and Rudolf Amann |
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| Institution: | Enric Llobet-Brossa, Ramon Rosselló-Mora, and Rudolf Amann |
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| Abstract: | The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4′,6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belonging to known phyla. Members of the Cytophaga-Flavobacterium cluster were most abundant in all layers, followed by the sulfate-reducing bacteria. |
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