麦麸及其挥发性物质对二点委夜蛾幼虫的引诱作用

【目的】二点委夜蛾Athetis lepigone是我国夏玉米上的一种新害虫，对玉米苗危害严重。由于其幼虫常隐蔽在收割机收获小麦后留在田间的麦秸和麦糠下，防治非常困难，因此开展二点委夜蛾幼虫引诱剂的研究具有重要的意义。【方法】本实验用“Y”型嗅觉仪测定了二点委夜蛾幼虫对麦秆﹑麦糠﹑玉米苗﹑麦麸﹑炒麦麸5种试材的行为反应；利用顶空固相微萃取(HS-SPME)与气相色谱 质谱联用仪（GC-MS）分析，比较了炒麦麸、麦麸中挥发物的成分，并从中挑选出6种化合物单体、8种混合物进行室内诱集实验。【结果】5种试材中炒麦麸引诱效果最好。6种单体中， 2-庚酮在5 μg/μL浓度下的引诱效果最好，引诱率为75.9%。8种混合物中，2 庚酮+苯甲醛+对二甲苯的引诱效果较好，引诱率为72.7%，但引诱效果不如2 庚酮单体。【结论】结果说明，2-庚酮对二点委夜蛾幼虫有强烈的引诱作用，从而为二点委夜蛾幼虫的田间诱杀技术提供了研究基础。

Attractiveness of wheat bran and its volatiles to larvae of Athetis lepigone (Lepidoptera: Noctuidae)

【Aim】 Athetis lepigone (Möschler) (Lepidoptera: Noctuidae) is a new insect pest in summer corn and causes serious damages to corn seedlings in China. It is very difficult to control this insect pest because its larvae hide beneath the straw and bran of winter corn in the field after harvest by combines. So it is important to study the attractant for A. lepigone larvae. 【Methods】 Y-tube olfactometer tests were conducted to investigate taxis responses of A. lepigone larvae to straw, bran, wheat bran, fried wheat bran and corn seedlings. The volatile components of fried wheat bran and wheat bran were analyzed by HS-SPME and GC-MS, and six compounds and eight blends were selected to further conduct the laboratory trapping. 【Results】 Among the five materials, fried wheat bran was the most attractive to A. lepigone larvae, and among the six volatile components, 2-heptanone (5 μg/μL) showed the strongest attractiveness to A. lepigone larvae, with the luring rate of 75.9%. Among eight blends, a three-component blend (2-heptanone＋benzaldehyde＋p-xylene) strongly attracted A. lepigone larvae with the luring rate of 72.7%, but its attractiveness effect was lower than that of 2-heptanone. 【Conclusion】 The results showed that 2 heptanone has the strongest attractiveness to A. lepigone larvae. These results provide a basis for controlling the larvae of A. lepigone with field trapping.