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A flat-coil NMR probe with hydration control of oriented phospholipid bilayer samples |
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| Authors: | N C Nielsen P Daugaard V Langer J K Thomsen S Nielsen O W Sørensen H J Jakobsen |
| Institution: | (1) Department of Chemistry, University of Aarhus, DK-8000 Aarhus C, Denmark;(2) Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus C, Denmark;(3) Novo Nordisk A/S, DK-2880 Bagsværd, Denmark |
| Abstract: | Summary A novel flat-coil solid-state NMR probe capable of controlling the hydration of oriented phospholipid bilayers in the course of long-term experiments, is described. Perfect hydration control for at least five days of intense radio-frequency pulsing is demonstrated using 31P NMR of oriented dimyristoylphospha-tidylcholine bilayers. The probe design will be of particular importance for studies of peptides and proteins oriented in lipid bilayers. |
| Keywords: | Flat-coil probe Solid-state NMR Oriented lipid bilayer Hydration control 31P chemical shift |
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