Intracellular calcium antagonist protects cultured peritoneal macrophages against anthrax lethal toxin-induced cytotoxicity |
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Authors: | S Shin G-H Hur Y-B Kim K-J Park Y-M Park W-S Lee |
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Institution: | CBR Department, Agency for Defense Development, Yuseong, Taejon. |
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Abstract: | The lethal toxin ofBacillus anthracis is central to the pathogenesis of anthrax. Using primary cultures of mouse peritoneal macrophages, we have demonstrated that
intracellular calcium release inhibitors protect against anthrax lethal toxin-induced cytotoxicity. The cytolytic effect of
anthrax lethal toxin was markedly reduced by dantrolene, an inhibitor of calcium release from intracellular calcium stores.
Pretreatment of macrophages with cyclosporin A, which has been shown to be a potent inhibitor of calcium release from mitochondria,
also protected cells against cytotoxicity. These results indicate that calcium release from intracellular store may be an
essential step for the propagation of anthrax lethal toxin-induced cell damage in macrophages. Thus our findings suggest that
dantrolene, cyclosporin A, and possibly other drugs affecting intracellular calcium pools might be effectively preventing
the toxicity from anthrax lethal toxin.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | anthrax lethal toxin cytotoxicity intracellular calcium antagonist macrophage |
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