Derivation,characterization and expansion of fetal chondrocytes on different microcarriers |
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Authors: | Gaye Çetinkaya An?l Sera Kahraman Menem?e Gümü?derelio?lu Sezen Arat Mehmet Ali Onur |
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Institution: | (1) TUBITAK MRC-Genetic Engineering Biotechnology Institute (GEBI), 41470 Gebze/Kocaeli, Turkey;(2) Chemical Engineering and Bioengineering Departments, Hacettepe University, 06800 Beytepe, Ankara, Turkey;(3) Faculty of Science, Department of Biology, Hacettepe University, 06800 Beytepe, Ankara, Turkey; |
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Abstract: | Fetal chondrocytes (FCs) have recently been identified as an alternative cell source for cartilage tissue engineering applications
because of their partially chondrogenically differentiated phenotype and developmental plasticity. In this study, chondrocytes
derived from fetal bovine cartilage were characterized and then cultured on commercially available Cytodex-1 and Biosilon
microcarriers and thermosensitive poly(hydroxyethylmethacrylate)-poly(N-isopropylacrylamide) (PHEMA-PNIPAAm) beads produced
by us. Growth kinetics of FCs were estimated by means of specific growth rate and metabolic activity assay. Cell detachment
from thermosensitive microcarriers was induced by cold treatment at 4 °C for 20 min or enzymatic treatment was applied for
the detachment of cells from Cytodex-1 and Biosilon. Although attachment efficiency and proliferation of FCs on PHEMA-PNIPAAm
beads were lower than that of commercial Cytodex-1 and Biosilon microcarriers, these beads also supported growth of FCs. Detached
cells from thermosensitive beads by cold induction exhibited a normal proliferative activity. Our results indicated that Cytodex-1
microcarrier was the most suitable material for the production of FCs in high capacity, however, ‘thermosensitive microcarrier
model’ could be considered as an attractive solution to the process scale up for cartilage tissue engineering by improving
surface characteristics of PHEMA-PNIPAAm beads. |
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Keywords: | Fetal chondrocyte Thermosensitive polymers Microcarriers PNIPAAm Cartilage tissue engineering |
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