Cell growth optimization in microcarrier culture |
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Authors: | B Mered P Albrecht Hope E Hopps |
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Institution: | (1) Department of Health, Education, and Welfare, Public Healt Service, Food and Drug Administration, Bureau of Biologics, Division of Virology, 8800 Rockville Pike, 20205 Bethesda, Maryland |
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Abstract: | Summary Three monkey kidney cell lines and primary chicken embryo cells were grown in microcarrier culture. The carrier support was
DEAE-Sephadex gel beads at low anion exchange capacity prepared according to a protocol developed at the Massachusetts Institute
of Technology.
The growth rate of the cells and the final cell density in microcarrier culture was dependent on the concentration of the
beads in culture and on the size of the initial cell inoculum. A bead concentration of 1.0 to 2.0 mg of beads/ml of tissue
culture medium and a cell inoculum of 20,000 cells/cm2 of bead surface appeared to be optimal. The efficiency of the microcarrier culture system was compared to that of stationary
and roller bottle cultures. Stationary flasks gave cell densities about twofold higher than maximal densities in roller bottles
and about threefold and twofold higher than cell densities in microcarrier culture at a bead concentration of 2.5 and 1.0
mg/ml, respectively.
In terms of cell yield per millitier of tissue culture medium, the microcarrier culture was superior to roller bottle and
stationary cultures. An advantage of the microcarrier culture system is its suitability for a scale up into large volume production
units. |
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Keywords: | microcarrier culture monkey kidney cells primary chicken embryo cells |
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