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Analysis of mitochondrial DNA from somatic hybrid cell lines of Saccharum officinarum (sugarcane) and Pennisetum americanum (pearl millet)
Authors:Zohreh Tabaeizadeh  Daryl R. Pring  Indra K. Vasil
Affiliation:(1) Department of Botany, University of Florida, 32611 Gainesville, FL, USA;(2) USDA-ARS, Department of Plant Pathology, University of Florida, 32611 Gainesville, FL, USA;(3) Present address: Centre for Plant Molecular Biology, Department of Biology, McGill University, H3A 1B1 Montreal, Quebec, Canada
Abstract:Mitochondrial DNA (mtDNA) from cell suspension cultures of two intergeneric somatic hybrids of Pennisetum americanum (pearl millet) + Saccharum officinarum (sugarcane) was examined by restriction endonuclease digestion and hybridization with sorghum mtDNA cosmids. The mtDNA of one somatic hybrid was indistinguishable from that of pearl millet, while the second exhibited a combination of parental mtDNAs, suggesting mitochondrial fusion. Several novel, possibly recombinant, mtDNA restriction fragments were detected in this hybrid, which may have resulted from intergenmic recombination.Florida Agriculture Experiment Station Journal Series No: 8090.
Keywords:Gramineae  mitochondrial recombination  somatic hybrids
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