Evaluation of a commercial Erns-capture ELISA for detection of BVDV in routine diagnostic cattle serum samples |
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| Authors: | Jaruwan Kampa Karl Ståhl Lena HM Renström Stefan Alenius |
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| Affiliation: | (1) Department of Clinical Sciences, Swedish University of Agricultural Science (SLU), SE-75007 Uppsala, Sweden;(2) Faculty of Veterinary Medicine, Khon Kaen University, 40002, Thailand;(3) Department of Biomedical Sciences and Veterinary Public Health, SLU, SE-75007 Uppsala, Sweden;(4) National Veterinary Institute (SVA), SE-75007 Uppsala, Sweden |
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| Abstract: | Background Bovine viral diarrhoea virus (BVDV) is an important pathogen in cattle. The ability of the virus to cross the placenta during early pregnancy can result in the birth of persistently infected (PI) calves. These calves shed the virus during their entire lifespan and are the key transmitters of infection. Consequently, identification (and subsequent removal) of PI animals is necessary to rapidly clear infected herds from the virus. The objective of this study was to evaluate the suitability of a commercial Erns-capture ELISA, in comparison to the indirect immunoperoxidase test (IPX), for routine diagnostic detection of BVDV within a control programme. In addition, the effect of passive immunity and heat-inactivation of the samples on the performance of the ELISA was studied. |
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