Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between <Emphasis Type="Italic">in situ</Emphasis> electroporation and retroviral transduction |
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Authors: | Sébastien JD Giroux Celmar Alves-Leiva Yann Lécluse Patrick Martin Olivier Albagli Isabelle Godin |
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Institution: | 1.INSERM U790, Institut Gustave Roussy-PR1,Villejuif,France;2.Institut Gustave Roussy,Villejuif,France;3.Université de Paris XI,Orsay,France;4.CNRS UMR6548; Université Nice-Sophia Antipolis, Bat. Sciences Naturelles; Parc Valrose,Nice Cedex 2,France |
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Abstract: | Background Hematopoietic development in vertebrate embryos results from the sequential contribution of two pools of precursors independently
generated. While intra-embryonic precursors harbour the features of hematopoietic stem cells (HSC), precursors formed earlier
in the yolk sac (YS) display limited differentiation and self-renewal potentials. The mechanisms leading to the generation
of the precursors in both sites are still largely unknown, as are the molecular basis underlying their different potential.
A possible approach to assess the role of candidate genes is to transfer or modulate their expression/activity in both sites.
We thus designed and compared transduction protocols to target either native extra-embryonic precursors, or hematopoietic
precursors. |
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