水稻谷蛋白GluA-2基因在小麦胚乳中的表达

水稻(Oryza sativa L.)谷蛋白(Glutelin)约占水稻储藏蛋白总量的80%,谷蛋白赖氨酸含量较高并易于被人体消化吸收.为了提高小麦(Triticum aestivum L.)的营养品质,将水稻谷蛋白GluA-2基因的cDNA序列导人小麦栽培品种Bobwhite(T. aestivum cv.Bobwhite).共轰击了600个小麦幼胚,经PCR和Southern杂交鉴定,共获得4棵转GluA-2基因小麦;SDS-PAGE分析表明,GluA-2基因在3棵转基因植株及其后代中表达,在1棵转基因植株中未表达,但其内源的高分子量麦谷蛋白亚基Bx7和By9含量显著降低,并且可遗传至T1代.

Expression of Rice Glutelin Gene GluA-2 in Wheat Endosperm

Rice (Oryza sativa L.) glutelin accounts for about 80% of total seed storage protein. Glutelin can be easily digested by human and contains high concentrations of lysine and other essential amino acids. To improve the nutritional quality of wheat (Triticum aestivum L.), the cDNA sequence of rice glutelin gene GluA-2 was introduced into common wheat through biolistic bombardment. Six hundreds of immature embryos from wheat cultivar Bobwhite (T. aestivum cv. Bobwhite) were bombarded. Four transgenic plants carrying GluA-2 gene were confirmed by PCR and Southern blotting analyses. Gene GluA-2 was translated in three of the transgenic plants and their progeny, as indicated by SDS-PAGE analyses. The expression of GluA-2 gene was not detected in one transgenic plant and the endogenous wheat high molecular weight glutenin subunits Bx7 and By9 contents decreased greatly in both T₀ and T₁ generations.