Biochemical and Genetic Evidence for Participation of DevR in a Phosphorelay Signal Transduction Pathway Essential for Heterocyst Maturation in Nostoc punctiforme ATCC 29133 |
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Authors: | Kari D. Hagen and John C. Meeks |
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Affiliation: | Section of Microbiology, Division of Biological Sciences, University of California, Davis, California 95616, USA. |
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Abstract: | In a test of the hypothesis that DevR is a response regulator protein that functions in a phosphorelay signal transduction system involved in heterocyst development in Nostoc punctiforme ATCC 29133, purified affinity-tagged DevR was shown to be phosphorylated in vitro by the noncognate sensor kinase EnvZ. Site-directed mutagenesis was used to generate N. punctiforme mutants with single amino acid substitutions at the putative phosphorylation site of DevR. These mutants exhibited a Fox- phenotype like the original devR insertion mutant UCD 311, consistent with a phosphotransferase role for DevR. |
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