Isolation and mapping of a uracil-sensitive mutant of Salmonella typhimurium |
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| Authors: | Lee B Bussey and John L Ingraham |
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| Institution: | (1) Department of Bacteriology, University of California, 95616 Davis, California, USA;(2) Present address: Department of Biology, Georgia State University, University Plaza, 30303 Atlanta, Georgia, USA |
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| Abstract: | Summary A uracil-sensitive mutant of Salmonella typhimurium was isolated by diethyl sulfate mutagenesis and penicillin counterselection. This mutation identifies a new Salmonella gene that is well separated from the structural genes for arginine and pyrimidine biosynthesis. The use-1 mutation was located between the ilv gene cluster (isoleucine-valine operon) and hisR (structural gene for histidine tRNA) at 83 map units. |
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