NAD-dependent inhibition of the NAD-glycohydrolase activity in A549 cells |
| |
Authors: | Balducci Enrico Micossi Luigi G. |
| |
Affiliation: | Dipartimento di Scienze Morfologiche e Biochimiche Comparate, Universita' degli Studi di Camerino, Italy. enrico-balducci@durou.it |
| |
Abstract: | NAD glycohydrolases are enzymes that catalyze the hydrolisis of NAD to produce ADP-ribose and nicotinamide. Regulation of these enzymes has not been fully elucidated. We have identified an NAD-glycohydrolase activity associated with the outer surface of the plasma membrane in human lung epithelial cell line A549. This activity is negatively regulated by its substrate -NAD but not by -NAD. Partial restoration of NADase activity after incubation of the cells with arginine or histidine, known ADP-ribose acceptors, suggests that inhibition be regulated by ADP-ribosylation. A549 do not undergo to apoptosis upon NAD treatment indicating that this effect be likely mediated by a cellular component(s) lacking in epithelial cells. |
| |
Keywords: | NAD-glycohydrolase epithelial cells ectoenzyme ADP-ribosylation NAD |
本文献已被 PubMed SpringerLink 等数据库收录! |