| A型猪流感病毒山东分离株鉴定及其HA基因序列分析 |
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| 引用本文： | 许传田,范伟兴,赵宏坤.A型猪流感病毒山东分离株鉴定及其HA基因序列分析[J].Virologica Sinica,2004,19(1):27-31. |
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| 作者姓名： | 许传田 范伟兴 赵宏坤 |
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| 作者单位： | 山东农业大学动物科技学院 山东泰安271018
(许传田，范伟兴)，山东农业大学动物科技学院 山东泰安271018(赵宏坤) |
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| 摘 要： | 从山东各地疑似流感发病猪分离到10株流感病毒，经国家流感中心鉴定均为A型流感病毒H9N2亚型。将其中一株Sw／SD／1／2003(H9N2)的血凝素全基因(HA)进行克隆与测序，与GenBank收录的其它猪流感和禽流感H9N2亚型的HA基因进行比较，发现Sw／SD／1／2003(H9N2)的血凝素基因在核苷酸序列方面同广西1999年分离的禽流感毒株Ck／GX／99(H9N2)和2000年云南分离的禽流感毒株Ck／YN／2000(H9N2)的同源性最高；进化树分析表明Sw／SD／1／2003(H9N2)起源于禽源的H9N2亚型流感病毒；Sw／SD／1／2003的HA氨基酸裂解位点与其他H9N2亚型不问，Sw／SD／1／2003的HA氨基酸裂解位点是R-S-L-R-G，而其它猪流感和禽流感H9N2亚型都是R-S-S-R-G。
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| 关 键 词： | 猪流感病毒 氨基酸裂解化点 HA基因 序列分析 |
Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene |
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| XU Chuan-tian,FAN Wei-xing,ZHAO Hong-kun.Isolation and Identification of Type A Swine Influenza Virus from Shandong Province and Sequence Analysis of HA Gene[J].中国病毒学(英文版),2004,19(1):27-31. |
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| Authors: | XU Chuan-tian FAN Wei-xing ZHAO Hong-kun |
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| Institution: | XU Chuan-tian,FAN Wei-xing**,ZHAO Hong-kun |
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| Abstract: | influenza viruses were isolated from sicked pigs in different places of Shandong province from autumn of 2002 to spring of 2003. They were all identified as Influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA gene of one isolate Sw/SD/1/2003 (H9N2) was cloned and sequenced and compared with other H9N2 isolates of avian and swine in GenBank. The results indicated that the isolate Sw/SD/1/2003 (H9N2) was similar to Ck/GX/1999 (H9N2) and Ck/YN/2000 (H9N2 ) in HA gene; Sw/SD/1/2003 might come from an avian influenza H9N2 subtype according to the phylogenetic analysis of HA genes; the amino acid sequence at the HA cleavage site in Sw/SD/1/2003 is R-S-L-R-G, but the sequences of all other H9N2 subtypes of porcine isolates and avian isolates are R-S-S-R-G. |
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| Keywords: | Swine influenza virus Sw/SD/1/2003 HA Amino acid cleave site |
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