Functional characterization of an androgen response element in the first intron of the C3(1) gene of prostatic binding protein |
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| Authors: | F Claessens L Celis B Peeters W Heyns G Verhoeven W Rombauts |
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| Institution: | Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Belgium. |
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| Abstract: | We demonstrate that the 204 bp intronic gene fragment of C3(1), which has a specific in vitro affinity for the androgen receptor, is able to confer androgen responsiveness to a heterologous promoter. This characteristic is completely destroyed by a single G----T substitution, affecting a 5'-TGTTCT-3' element that closely resembles the consensus sequence of the glucocorticoid and progesterone response elements (GRE/PRE). In fact we could show that this androgen response element (ARE) also acts as a similarly weak GRE or PRE in T-47D cells. |
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