Induced regulatory T-cells (iTregs) generated by activation with anti-CD3/CD28 antibodies differ from those generated by the physiological-like activation with antigen/APC |
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Authors: | Chan Zhao Guangpu Shi Barbara P. Vistica Samuel J.H. Hinshaw Wambui S. Wandu Cuiyan Tan Meifen Zhang Igal Gery |
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Affiliation: | 1. Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, United States;2. Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China |
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Abstract: | Regulatory T-cells (Tregs) are responsible for homeostasis of the immune system, as well as for inhibition of pathogenic autoimmune processes. Induced-(i)-Tregs, can be generated in vitro by activation of CD4 cells in the presence of TGF-β. A commonly used activation mechanism is by antibodies against CD3 and CD28. The physiological-like activation of T-cells, however, is with the specific target antigen presented by antigen-presenting cells (APC). The two modes of activation have been considered to yield the same populations of iTregs. Here, we compared between iTreg populations generated by either one of the two methods and found differences between their capacities to inhibit T-lymphocyte proliferative response, their expression of cell surface antigens and particularly, in their transcript expression profiles of certain chemokines and chemokine receptors. Our data thus indicate that iTregs generated by activation with anti-CD3/CD28 antibodies cannot be considered identical to iTregs generated by antigen/APC. |
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Keywords: | Treg, regulatory T cells Foxp3, fork head box P3 nTregs, natural Tregs iTregs, induced Tregs TCR, T cell receptor Tg, transgenic APC, antigen presenting cell Th, T-helper HEL, hen egg lysozyme CD, cluster of differentiation HA, activated by HEL and APC PbAb, activated by plate-bound anti-CD3/CD28 antibodies CCR, CC chemokine receptor CXCR, CXC chemokine receptor LFA-1, lymphocyte function-associated antigen 1 PSGL-1, P-selectin glycoprotein ligand 1 |
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