Cord blood CD4CD25 regulatory T cells fail to inhibit cord blood NK cell functions due to insufficient production and expression of TGF-beta1 |
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| Authors: | Liqing Xu Shigeki Tanaka Motoki Bonno Masaru Ido Masatoshi Kawai Hatsumi Yamamoto Yoshihiro Komada |
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| Affiliation: | 1. Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan;2. Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan;3. Shanghai Children’s Medical Center, Shanghai, China;4. Department of Psychology and Social Welfare, School of Letters, Mukogawa Women’s University, Nishinomiya, Hyogo, Japan;5. Department of Pediatrics, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan |
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| Abstract: | Although CD4+CD25+ Treg (Treg) cells are known to modulate NK cell functions, the modulation mechanism of these cells in cord blood has not been fully clarified. The purpose of this study was to clarify the mechanism whereby cord blood Treg cells modulate cord NK cells. By performing various cultures of purified NK cells with or without autologous Treg cells, diminished inhibitory effects of cord Treg cells towards cord NK cell functions, including activation, cytokine production, and cytotoxicity, were observed. We also observed lower secretion of sTGF-beta1 and lower expression of mTGF-beta1 by cord Treg cells than by adult Treg cells. These data revealed the capability of adult Treg cells to suppress rhIL-2-stimulated NK cell function by TGF-beta1, both membrane-bound and soluble types. The reduced inhibitory capabilities of cord Treg cells compared with adult Treg cells is thought to be due to insufficient expression of TGF-beta1. |
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| Keywords: | Treg cell, CD4+CD25+ regulatory T cell NK cell, natural killer cell PBMCs, peripheral blood mononuclear cells CBMCs, cord blood mononuclear cells MLC, mixed lymphocyte culture FOXP3, forkhead box P3 rhIL-2, recombinant human IL-2 rhTGF-beta1, recombinant human transforming growth factor beta1 mTGF-beta1, membrane-bound transforming growth factor-beta1 sTGF-beta1, soluble transforming growth factor-beta1 |
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