首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Interleukin-17F attenuates H2O2-induced cell cycle arrest
Authors:Zan Tong  Huichao Yan  Weihuang Liu
Institution:1. School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China;2. Zhongshan Hospital of Hubei Province, Wuhan 430033, China
Abstract:Interleukin IL-17F was expressed in colon epithelial cells and showed multiple functions in colon tumorigenesis. However, the role of IL-17F in colon cancer cell cycle progression remains unclear. In this study, we analyzed the effects of IL-17F on oxidant-induced cell cycle shift in human colon cancer cells. IL-17F overexpressing and wildtype HCT116 cells were challenged with H2O2. Cell cycle distribution analysis showed IL-17F attenuated H2O2-induced G2/M phase arrest by inhibiting S to G2/M transition. We further checked expression levels of two critical cell cycle regulators p21 and p27. The results showed that IL-17F could inhibit H2O2 induced p27 up-regulation. Meanwhile, IL-17F could increase the phosphorylation of p38 after H2O2 treatment. The regulations of p27 level and p38 activity may contribute to the impaired G2/M phase arrest by IL-17F. Taken together, our findings extend IL-17F as an important factor in colon cancer development and provide new insight into the signaling pathway.
Keywords:FACS  fluorescence-activated cell sorting
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号