Super-resolution Microscopy Approaches for Live Cell Imaging |
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| Authors: | Antoine G Godin Brahim Lounis Laurent Cognet |
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| Institution: | 1 University of Bordeaux, LP2N, UMR 5298, Talence, France;2 Institut d’Optique Graduate School and Centre National de la Recherche Scientifique, LP2N, UMR 5298, Talence, France |
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| Abstract: | By delivering optical images with spatial resolutions below the diffraction limit, several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nanoscale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells. |
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