Design and construction of a preparative-scale dynamic field gradient focusing apparatus |
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Authors: | Tracy Noah I Huang Zheng Ivory Cornelius F |
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Affiliation: | School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA. |
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Abstract: | A linear model is used to show that dynamic field gradient focusing (DFGF) can be scaled to preparative capacity, approximately O (10 mgs). This paper explains how the preparative-scale DFGF apparatus was designed and fabricated. Scaled-down experiments and mathematical modeling guided material selection and design changes during construction to increase the probability that the prototype preparative-scale DFGF apparatus would perform as intended. The finished prototype successfully focused bovine hemoglobin from an initial concentration of 6.82 to 15 mg/mL and allowed for 86% recovery of injected protein. |
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