Fuzzy Logic Control of Bioreactor for Enhanced Biosynthesis of Alkaline Protease by an Alkalophilic Strain of Bacillus subtilis |
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Authors: | Ikram ul-Haq Hamid Mukhtar |
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Institution: | (1) Biotechnology Research Centre, Botany Department, Government College University, Lahore, 54000, Pakistan |
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Abstract: | Present studies describe the optimization of some cultural parameters such as medium pH, incubation temperature, and agitation
rate for the biosynthesis of alkaline protease by Bacillus subtilis IH-72 in a bioreactor using fuzzy logic control. The process of fermentation was carried out in a 7.5-L bioreactor (New Brunswick
Scientific, USA) with a working volume of 5 L. All of the parameters were automatically controlled with the help of attached
software. The optimum pH, temperature, and agitation for the production of alkaline protease by B. subtilis IH-72 were found to be 9.0, 35°C, and 175 rpm, respectively. The performance of the fuzzy logic of the bioreactor was found
to be encouraging for enhanced production of the enzymes. The maximum production of alkaline protease during the present study
was found to be 9.6 U mL−1. |
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