Hemin-incorporated nanoflowers as enzyme mimics for colorimetric detection of foodborne pathogenic bacteria |
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| Authors: | Kui-Yu Wang Sheng-Jun Bu Chuan-Jing Ju Chang-Tian Li Zhong-Yi Li Ye Han Cheng-You Ma Cheng-Yu Wang Zhuo Hao Wen-Sen Liu Jia-Yu Wan |
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| Institution: | 1. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China;2. The General Hospital of FAW, Changchun 130011, China;3. The Fourth Hospital of Jilin University, Changchun 130011, China;4. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China;5. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China;6. College of Geo-Exploration Science and Technology, Jilin University, Changchun 130026, China |
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| Abstract: | Rapid, sensitive and point-of-care detection of foodborne pathogenic bacteria is essential for food safety. In this study, we found that hemin-concanavalin A hybrid nanoflowers (HCH nanoflowers), as solid mimic peroxidase, could catalyze oxidation of 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) in the presence of H2O2 to a green-colored product. HCH nanoflowers, integrating the essential functions of both biological recognition and signal amplification, meet the requirements of signal labels for colorimetric immunoassay of bacteria. In view of the excellent peroxidase mimetic catalytic activity of HCH nanoflowers, a colorimetric biosensing platform was newly constructed and applied for sensitive detection of foodborne Escherichia coli O157:H7 (E. coli O157:H7). The corresponding detection limits was as low as 4.1?CFU/mL with wide linear ranges (101–106?CFU/mL). |
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| Keywords: | Hybrid nanoflowers Enzyme mimics Colorimetric detection Pathogenic bacteria |
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