Recent enhancements in SSCP |
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| Institution: | 1. Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamontol Sai 4, Salaya 73170, Nakorn Pathom, Thailand;2. Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand;3. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 111 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang 12120, Pathum Thani, Thailand;1. Divisions of Research, Atlanta, GA, United States;2. Maternal Fetal Medicine, Atlanta, GA, United States;3. Department of Gynecology and Obstetrics, Atlanta, GA, United States;4. Emory University School of Medicine, Atlanta, GA, United States |
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| Abstract: | High sensitivity, robustness and scalability are the three criteria which influence whether techniques for rapid mutation detection will be used in the future. PCR-SSCP, one of the most popular methods for detecting mutation, especially in the field of medical genetics, is being improved (1) to efficiently detect mutations in long stretches of PCR products; (2) to simplify data interpretation by removing PCR artifacts and (3) to minimize human involvement in the process of mutation detection by a simple post-PCR fluorescence labeling followed by separation using automated DNA sequencers. |
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