Natural Hybrids between Ligularia vellerea and L.subspicata (Asteraceae: Senecioneae) |
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Authors: | YU Jiao-Jun PAN Li PAN Yue-Zhi GONG Xun |
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Institution: | 1.Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, ;
Kunming 650201, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Yunnan Academy of Forestry, Kunming 650201, China |
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Abstract: | Three types of intermediate morphological individuals were found in the sympatric distribution areas of Ligularia vellerea and Lsubspicata. These intermediate individuals were hypothesized to be the hybrids of these two species based on the detailed comparison of their morphological characters. To test this hypothesis, we compared the DNA sequence of Lvellerea, Lsubspicata and the intermediate individuals by using the internal transcribed spacer region of nuclear ribosomal and atpB rbcL intergenic spacer region of the chloroplast. Direct sequencing of ITS clearly had additives and then cloned sequencing cloned out two kinds of sequences that were identical to those putative parental species, thus these putative hybrids were approved. With different morphological characters among three types, their plastid donors were all Lsubspicata according to the maternal sequences of atpB rbcL. |
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Keywords: | Ligularia vellerea L subspicata Hybrid progeny ITS atpB-rbcL |
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