Differential sequencing with mass spectrometry |
| |
Institution: | Center for Advanced Biotechnology, Boston University, Cummington Street, Boston MA 02215, USA |
| |
Abstract: | Differential or genetic sequencing requires searching sample DNA for variations with respect to a reference sequence. Conventional detection techniques are too labor and cost expensive for use in diagnostic applications, therefore new technologies will be required. Measurement techniques based on mass spectrometry (MS) possess the potential for high-throughput, high fidelity measurement of sequence variation. Unambiguous detection of polymorphic sequences has been demonstrated, even in heterozygous samples. Automated reproducible measurements of microscopic arrays of samples will enable the high-throughput detection required for large-scale applications. Computational simulation and analysis of experimental parameters prior to experimentation will provide the optimization necessary for development of robust, reproducible measurements. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|