FLASH-PCR diagnostics of toxigenic fungi of the genus <Emphasis Type="Italic">Fusarium</Emphasis> |
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| Authors: | D Yu Ryazantsev S L Abramova S V Evstratova T Yu Gagkaeva S K Zavriev |
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| Institution: | (1) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia;(2) All-Russia Institute of Phytopathology, Russian Academy of Agricultural Sciences, pos. Bol’shie Vyazemy, Moscow oblast, 143050, Russia;(3) All-Russia Institute of Plant Protection, Russian Academy of Agricultural Sciences, Pushkin, St. Petersburg, 196608, Russia |
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| Abstract: | A test system for the diagnostics and identification of seven toxigenic fungi causing Fusarium head blight (Fusarium graminearum, F. culmorum, F. poae, F. sporotrichioides, F. langsethiae, F. avenaceum, and F. tricinctum) was developed using PCR. The identification of pathogen is based on the specific amplification of a DNA fragment of the gene of translation elongation factor 1 alpha (tef-1α) and subsequent detection of the results by the fluorescent amplification-based specific hybridization method. The system was tested on 38 isolates of different fungi of the genus Fusarium. |
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| Keywords: | polymerase chain reaction diagnosis mycotoxin fusariosis phytopathogen |
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