Isolation of Enterococcus hirae mutant deficient in low-affinity potassium uptake at alkaline pH |
| |
Authors: | Kawano Miyuki Igarashi Kazuei Kakinuma Yoshimi |
| |
Institution: | Graduate School of Pharmaceutical Sciences, Chiba University, Japan. |
| |
Abstract: | We here isolated an Enterococcus hirae mutant unable to grow well at pH 10. The influx rate calculated from steady-state 42K+/K+ exchange and the intracellular K+ concentration of the mutant were reduced to 53 and 55% of those of the wild-type, respectively. The activities of two high-affinity K+ uptake systems, KtrI and KtrII, were normal in the mutant, but the kinetics of net K+ uptake at pH 10 indicated that a low-affinity K+ uptake with a Km of about 20 mM (Kawano, M, Abuki, R, Igarashi, K, Kakinuma, Y. (2001) Arch. Microbiol. 175: 41-45), which were seen in the wild-type, was deficient in this mutant. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|