Identity tests: determination of cell line cross-contamination |
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| Authors: | C M Cabrera F Cobo A Nieto J L Cortés R M Montes P Catalina A Concha |
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| Institution: | (1) Stem Cell Bank of Andalucia (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avenida de las Fuerzas Armadas No. 2, 18014 Granada, Spain |
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| Abstract: | Cell line cross-contamination is a phenomenon that arises as a result of the continuous cell line culture. It has been estimated that around 20% of the cell lines are misidentified, therefore it is necessary to carry out quality control tests for the detection of this issue. Since cell line cross-contamination discovery, different methods have been applied, such as isoenzyme analysis for inter-species cross-contamination; HLA typing, and DNA fingerprinting using short tandem repeat and a variable number of tandem repeat for intra-species cross-contamination. The cell banks in this sense represent the organizations responsible for guaranteeing the authenticity of cell lines for future research and clinical uses. |
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| Keywords: | Cell lines Cross-contamination Fingerprinting Isoenzyme analysis Short tandem repeat Variable number tandem repeat |
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