A semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process |
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Authors: | Aumann Lars Morbidelli Massimo |
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Affiliation: | ETH Zurich, Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, Hoenggerberg, HCI, 8093 Zurich, Switzerland. |
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Abstract: | The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better performance than the original 6-column process due to an additional degree of freedom, that is a different switching time for the "batch lane" and the "interconnected lane." Experimental results for the 3-column MCSGP unit of the purification of an industrial multicomponent peptide mixture containing 46% of Calcitonin on a reversed phase resin are compared with model simulations. It is concluded, that the model is well suited to predict the system behavior and therefore to design its optimal operating conditions. |
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Keywords: | peptide purification MCSGP SMB three‐fraction separation countercurrent solvent gradient semicontinuous Calcitonin biochromatography |
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