| 利用改进的酚-氯仿法从猪毛囊中提取基因组DNA |
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| 引用本文： | 王继英,俞英,冯利霞,王怀中,张勤.利用改进的酚-氯仿法从猪毛囊中提取基因组DNA[J].遗传,2010,32(7):752-756. |
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| 作者姓名： | 王继英 俞英 冯利霞 王怀中 张勤 |
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| 作者单位： | 1. 中国农业大学动物科技学院, 北京100193; 2. 山东省农业科学院畜牧兽医研究所, 济南 250100 |
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| 基金项目： | 国家转基因重大专项，国家重点基础研究发展规划(973计划)项目，国家高技术研究发展计划项目(863计划)，国家自然科学基金项目 |
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| 摘 要： | 为提高从猪毛囊组织中提取基因组DNA的效率, 文章在借鉴从其他组织提取基因组DNA方法的基础上, 对经典的酚-氯仿法的反应体系和步骤进行了改进。利用改进的酚－氯仿抽提法, 从猪的毛囊组织中快速、高效地提取了高质量基因组DNA。利用该方法从1~6根猪毛囊中提取的基因组DNA可满足基于PCR技术的相关分子生物学实验需要。
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| 关 键 词： | 猪毛囊组织 基因组DNA 酚-氯仿法 |
| 收稿时间： | 2009-12-28 |
Genomic DNA extraction from hair sacs of pigs using modified phenol-chloroform method |
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| WANG Ji-Ying,YU Ying,FENG Li-Xia,WANG Huai-Zhong,ZHANG Qin.Genomic DNA extraction from hair sacs of pigs using modified phenol-chloroform method[J].Hereditas,2010,32(7):752-756. |
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| Authors: | WANG Ji-Ying YU Ying FENG Li-Xia WANG Huai-Zhong ZHANG Qin |
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| Institution: | 1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan 250100, China |
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| Abstract: | In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol–chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol–chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with satisfied quantity and quality for the need of PCR-based molecular ex-periment. |
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| Keywords: | pig hair sac genomic DNA extraction phenol-chloroform method |
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